How old is the Indo-European language family? A biological solution to a linguistic problem. Quentin D. Atkinson & Russell D. Gray Department of Psychology, University of Auckland, Private Bag 92019, Auckland 1020, NEW ZEALAND E-mail: - qatk001@ec.auckland.ac.nz Languages, like genes, provide vital clues about human history (Gray & Jordan, 2000). The origin of the Indo-European language family is 'the most intensively studied, yet still most recalcitrant, problem of historical linguistics' (Diamond & Bellwood, 2003). Due to slow rates of genetic change, admixture, and the relatively recent timescales involved, genetic analyses have not conclusively resolved debates about time-depth in Indo-European. Languages, however, change much faster than genes and so contain more historical information at shallower time-depths. Despite this, traditional means of linguistic analysis have also been unable to provide convincing evidence of Indo-European origins. This is primarily due to problems associated with variable rates of language evolution and an inability to quantify the degree of statistical uncertainty in estimated ages. Here, we explore the application of new phylogenetic date estimation techniques to linguistic data. These methods are able to estimate divergence times and the uncertainty associated with each estimate, even under conditions of rate heterogeneity. We combine maximum-likelihood models of language evolution, Bayesian inference of phylogeny and rate smoothing algorithms to test between two theories of Indo-European origin - the 'Kurgan expansion' and 'Anatolian farming' hypotheses. The Kurgan hypothesis centres on possible archaeological evidence for an expansion into Europe and the near-East by Kurgan horsemen beginning in the sixth millennium BP (Gimbutas, 1973). The Anatolian hypothesis claims that Indo-European languages expanded with the spread of agriculture from Anatolia around 8,000 to 9,500BP (Renfrew, 1987). In striking agreement with the Anatolian hypothesis, our analysis of a matrix of 87 languages with 2,449 lexical items produced an estimated age range for the initial Indo-European divergence of between 7,800BP and 9,800BP (Gray & Atkinson, In press). The results were robust to changes in coding procedures, calibration points, rooting of the trees and priors in the Bayesian analysis. Diamond, J. & Bellwood, P. Farmers and Their Languages: The First Expansions. Science 300, 597 (2003). Gimbutas, M. The beginning of the Bronze Age in Europe and the Indo-Europeans 3500-2500 B.C. Journal of Indo-European Studies 1, 163-214 (1973). Gray, R. D. & Atkinson, Q. D. Language-tree divergence times support the Anatolian theory of Indo-European origin. Nature (In press). Gray, R.D. & Jordan, F.M. Language trees support the express-train sequence of Austronesian expansion. Nature 405, 1052-1055 (2000). Renfrew, C. Archaeology and Language: the Puzzle of Indo-European origins. London: Jonathan Cape (1987).